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An extensive blood culture protocol, including prolonged incubation of cultures, for 215 patients believed to have had endocarditis yielded only 3 clinically relevant results. Twenty-four Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella (i.e., HACEK) organisms were recovered from standard 5-day blood cultures during the same time period. Specialized methods and not extended incubation times are recommended for recovery of fastidious agents of septicemia.

clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)

Prolonged Incubation and Extensive Subculturing Do Not Increase Recovery of Clinically Significant Microorganisms from Standard Automated Blood Cultures