Open AccessESAT‐6 and CFP‐10 in Clinical versus Environmental Isolates ofMycobacterium kansasii
Author(s) -
Sandra M. Arend,
Petra de Haas,
Eliane M.S. Leyten,
Ida Rosenkrands,
Leen Rigouts,
Peter Andersen,
W. Mijs,
Jaap T. van Dissel,
Dick van Soolingen
Publication year - 2005
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/428950
Subject(s) - mycobacterium kansasii , virulence , esat 6 , biology , mycobacterium , mycobacterium tuberculosis , microbiology and biotechnology , gene , genotype , genetics , mycobacterium bovis , tuberculosis , virology , bacteria , medicine , bacterial protein , pathology
Mycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.
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