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To overcome some of the limitations of conventional microbiological techniques, polymerase chain reaction (PCR)-based assays have been proposed as a useful tool for the diagnosis of human brucellosis.

clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)

Comparison between LightCycler Real-Time Polymerase Chain Reaction (PCR) Assay with Serum and PCR-Enzyme-Linked Immunosorbent Assay with Whole Blood Samples for the Diagnosis of Human Brucellosis