Intracellular Macrophage Growth Rates and Cytokine Profiles ofMycobacterium tuberculosisStrains with Different Transmission Dynamics | Zendy

Sue A. Theus | Zendy; M. Donald Cave | Zendy; Kathleen D. Eisenach | Zendy

Open Access

Intracellular Macrophage Growth Rates and Cytokine Profiles ofMycobacterium tuberculosisStrains with Different Transmission Dynamics

Author(s) -

Sue A. Theus,

M. Donald Cave,

Kathleen D. Eisenach

Publication year - 2005

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/425936

Subject(s) - biology , mycobacterium tuberculosis , restriction fragment length polymorphism , microbiology and biotechnology , virulence , cytokine , tumor necrosis factor alpha , tuberculosis , intracellular , intracellular parasite , interferon gamma , virology , genotype , immunology , genetics , gene , medicine , pathology

Mycobacterium tuberculosis strains associated with IS6110 restriction fragment-length polymorphism (RFLP) pattern clusters and strains demonstrating unique IS6110 RFLP patterns were investigated in interferon- gamma -activated THP-1 cells by measurement of binding, intracellular growth rate, and cytokine production. Binding was the same for all strains; however, strains from clusters grew significantly more rapidly than did unique strains. Maximal concentration of tumor necrosis factor (TNF)-alpha was detected at 2 days after infection, with unique strains eliciting significantly greater amounts than did strains from clusters. Interleukin (IL)-10 levels peaked at 1 day after infection with strains from clusters, whereas they peaked at 5 days after infection with unique strains. Rapid growth demonstrated by strains from clusters was highly correlated with rapid production of IL-10 and suppression of TNF-alpha in THP-1 cells during the early stages of infection. Characterization of this phenotype will further advance the investigation of virulence factors in M. tuberculosis.

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