Codetection of a Mixed Population ofcandHPV62 Containing Wild‐Type and Disrupted E1 Open‐Reading Frame in a 45‐Year‐Old Woman with Normal Cytology | Zendy

Leiping Fu | Zendy; Masanori Terai | Zendy; Toshihiko Matsukura | Zendy; Rolando Herrero | Zendy; Robert D. Burk | Zendy

Open Access

Codetection of a Mixed Population ofcandHPV62 Containing Wild‐Type and Disrupted E1 Open‐Reading Frame in a 45‐Year‐Old Woman with Normal Cytology

Author(s) -

Leiping Fu,

Masanori Terai,

Toshihiko Matsukura,

Rolando Herrero,

Robert D. Burk

Publication year - 2004

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/423855

Subject(s) - orfs , open reading frame , biology , genetics , population , virology , cloning (programming) , genome , mutation , genital warts , microbiology and biotechnology , gene , peptide sequence , cervical cancer , cancer , medicine , environmental health , computer science , programming language

We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon.

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