Successful Induction of Protective Antibody Responses againstHaemophilus influenzaeType b and Diphtheria after Transcutaneous Immunization with the Glycoconjugate Polyribosyl Ribitol Phosphate–Cross‐Reacting Material197Vaccine | Zendy

Fatme Mawas | Zendy; Marisa Peyre | Zendy; AnneSophie Beig | Zendy; Laura S. Frost | Zendy; Giuseppe Del Giudice | Zendy; Rino Rappuoli | Zendy; Sylviane Muller | Zendy; Dorothea Sesardic | Zendy; Charalambos D. Partidos | Zendy

Open Access

Successful Induction of Protective Antibody Responses againstHaemophilus influenzaeType b and Diphtheria after Transcutaneous Immunization with the Glycoconjugate Polyribosyl Ribitol Phosphate–Cross‐Reacting Material₁₉₇Vaccine

Author(s) -

Fatme Mawas,

Marisa Peyre,

AnneSophie Beig,

Laura S. Frost,

Giuseppe Del Giudice,

Rino Rappuoli,

Sylviane Muller,

Dorothea Sesardic,

Charalambos D. Partidos

Publication year - 2004

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/423327

Subject(s) - diphtheria , diphtheria toxin , haemophilus influenzae , microbiology and biotechnology , immunization , glycoconjugate , hib vaccine , antibody , medicine , immunity , moraxella catarrhalis , cholera toxin , immunology , vaccination , virology , immune system , biology , conjugate vaccine , toxin , antibiotics , bioinformatics

We examined the antibody responses elicited in rats after transcutaneous immunization (TCI) with the Haemophilus influenzae type b (Hib)-cross-reacting material (CRM(197)) glycoconjugate vaccine coadministered with cholera toxin or mutants of heat-labile enterotoxin of Escherichia coli (LTK63 and LTR72) as adjuvants. The glycoconjugate vaccine was immunogenic, eliciting high antibody responses to the capsular polysaccharide of Hib and to diphtheria toxin. Passively transferred immune serum protected infant rats against challenge with the Hib Eagan strain and exhibited strong neutralizing activity against diphtheria toxin both in vitro and in vivo. The finding that TCI of rats can elicit antibody responses surpassing the minimum levels required for protective immunity against Hib and diphtheria suggests that this immunization strategy holds a lot of promise for future pediatric use. However, further studies are required to confirm the potential of TCI with glycoconjugate vaccines in humans.

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