Linezolid Resistance in SequentialStaphylococcus aureusIsolates Associated with a T2500A Mutation in the 23S rRNA Gene and Loss of a Single Copy of rRNA | Zendy

Venkata G. Meka | Zendy; Satish K. Pillai | Zendy; George Sakoulas | Zendy; Christine Wennersten | Zendy; Lata Venkataraman | Zendy; Paola C. DeGirolami | Zendy; George M. Eliopoulos | Zendy; Robert C. Moellering | Zendy; Howard S. Gold | Zendy

Open Access

Linezolid Resistance in SequentialStaphylococcus aureusIsolates Associated with a T2500A Mutation in the 23S rRNA Gene and Loss of a Single Copy of rRNA

Author(s) -

Venkata G. Meka,

Satish K. Pillai,

George Sakoulas,

Christine Wennersten,

Lata Venkataraman,

Paola C. DeGirolami,

George M. Eliopoulos,

Robert C. Moellering,

Howard S. Gold

Publication year - 2004

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/421471

Subject(s) - 23s ribosomal rna , linezolid , staphylococcus aureus , microbiology and biotechnology , biology , ribosomal rna , mutant , minimum inhibitory concentration , mutation , genotype , gene , genetics , bacteria , antibiotics , rna , vancomycin , ribosome

Linezolid is an important therapeutic option for infections caused by resistant gram-positive bacteria. We report the characterization of sequential methicillin-resistant Staphylococcus aureus (MRSA) bloodstream isolates that developed resistance in a patient treated with a prolonged course of linezolid. Analysis of this series of clinical MRSA isolates detected, in the resistant isolates, the presence of a T2500A mutation in the domain V region of the 23S rRNA gene. In addition, the loss of a single copy of the 23S rRNA gene was found in 2 of the resistant isolates. As a result of these 2 factors, the proportion of mutant : wild-type 23S rRNA genes increased in association with an increase in the minimum inhibitory concentration of linezolid. The most recent isolate of this series was recovered 7 months after the patient discontinued linezolid and demonstrated reversion to a susceptible phenotype associated with a loss of the T2500A mutation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research