Open AccessIsolation and Molecular Characterization of a Novel Type 3 Reovirus from a Child with Meningitis
Author(s) -
Kenneth L. Tyler,
Erik S. Barton,
M Ibach,
Christine C. Robinson,
Jacquelyn A. Campbell,
Sean O'donnell,
Tibor ValyiNagy,
Penny Clarke,
J. Denise Wetzel,
Terence S. Dermody
Publication year - 2004
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/383129
Subject(s) - virology , serotype , biology , meningitis , virus , strain (injury) , reoviridae , microbiology and biotechnology , capsid , gene , hemagglutination , isolation (microbiology) , hemagglutination assay , genome , genetics , medicine , rotavirus , titer , anatomy , psychiatry
Mammalian reoviruses are non-enveloped viruses that contain a segmented, double-stranded RNA genome. Reoviruses infect most mammalian species, although infection with these viruses in humans is usually asymptomatic. We report the isolation of a novel reovirus strain from a 6.5-week-old child with meningitis. Hemagglutination and neutralization assays indicated that the isolate is a serotype 3 strain, leading to the designation T3/Human/Colorado/1996 (T3C/96). Sequence analysis of the T3C/96 S1 gene segment, which encodes the viral attachment protein, sigma 1, confirmed the serotype assignment for this strain and indicated that T3C/96 is a novel reovirus isolate. T3C/96 is capable of systemic spread in newborn mice after peroral inoculation and produces lethal encephalitis. These results suggest that serotype 3 reoviruses can cause meningitis in humans.
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