Mapping Immune Reactivity toward Rv2653 and Rv2654: Two Novel Low‐Molecular‐Mass Antigens Found Specifically in theMycobacterium tuberculosisComplex | Zendy

Claus Aagaard | Zendy; Inger Brock | Zendy; Anja Weinreich Olsen | Zendy; Tom H. M. Ottenhoff | Zendy; Karin Weldingh | Zendy; Peter Andersen | Zendy

Open Access

Mapping Immune Reactivity toward Rv2653 and Rv2654: Two Novel Low‐Molecular‐Mass Antigens Found Specifically in theMycobacterium tuberculosisComplex

Author(s) -

Claus Aagaard,

Inger Brock,

Anja Weinreich Olsen,

Tom H. M. Ottenhoff,

Karin Weldingh,

Peter Andersen

Publication year - 2004

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/381679

Subject(s) - mycobacterium tuberculosis , tuberculosis , antigen , epitope , immune system , peripheral blood mononuclear cell , immunology , t cell , biology , virology , microbiology and biotechnology , interferon gamma , medicine , in vitro , biochemistry , pathology

New tools are urgently needed for the detection of latent tuberculosis (TB). We evaluated the diagnostic potential of 2 novel Mycobacterium tuberculosis complex-specific candidate antigens (Rv2653 and Rv2654) and investigated T cell recognition during natural infection in humans and experimental infection in guinea pigs. Peripheral blood mononuclear cells stimulated with peptide pools covering the full length of Rv2654 induced interferon- gamma release in 10 of 19 patients with TB. Neither Rv2654 single peptides nor Rv2654 pools were recognized by bacille Calmette-Guerin-vaccinated donors. However, peptides from Rv2653 were recognized by both patients group. The cross-reactive epitope(s) in Rv2653 were located in a 36-amino acid stretch in the center of the molecule. Rv2654 also induced M. tuberculosis-specific skin-test responses in 3 of 4 aerosol-infected guinea pigs. Rv2654 is a strongly recognized T cell antigen that is highly specific for TB and has potential as a novel cell-mediated immunity-based TB diagnostic agent.

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