Frequent Detection of Human Coronaviruses in Clinical Specimens from Patients with Respiratory Tract Infection by Use of a Novel Real‐Time Reverse‐Transcriptase Polymerase Chain Reaction | Zendy

Leontine J. R. van Elden | Zendy; Anton M. van Loon | Zendy; Floris van Alphen | Zendy; K. A. W. Hendriksen | Zendy; Andy I. M. Hoepelman | Zendy; Marian G.J. van Kraaij | Zendy; Jan Jelrik Oosterheert | Zendy; P Schipper | Zendy; Rob Schuurman | Zendy; Monique Nijhuis | Zendy

Open Access

Frequent Detection of Human Coronaviruses in Clinical Specimens from Patients with Respiratory Tract Infection by Use of a Novel Real‐Time Reverse‐Transcriptase Polymerase Chain Reaction

Author(s) -

Leontine J. R. van Elden,

Anton M. van Loon,

Floris van Alphen,

K. A. W. Hendriksen,

Andy I. M. Hoepelman,

Marian G.J. van Kraaij,

Jan Jelrik Oosterheert,

P Schipper,

Rob Schuurman,

Monique Nijhuis

Publication year - 2004

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/381207

Subject(s) - respiratory tract , real time polymerase chain reaction , polymerase chain reaction , reverse transcriptase , pneumonia , virology , medicine , covid-19 , reverse transcription polymerase chain reaction , respiratory system , biology , pathology , gene , disease , messenger rna , biochemistry , infectious disease (medical specialty)

During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens associated with more-severe respiratory tract infection (RTI). Diagnostic tests for HCoVs are not frequently used in the routine setting. It is likely that, as a result, the precise role that HCoVs play in RTIs is greatly underestimated. We describe a rapid, sensitive, and highly specific quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of HCoV that can easily be implemented in the routine diagnostic setting. HCoV was detected in 28 (11%) of the 261 clinical specimens obtained from patients presenting with symptoms of RTI ranging from common cold to severe pneumonia. Only 1 (0.4%) of the 243 control specimens obtained from patients without symptoms of RTI showed the presence of HCoV. We conclude that HCoVs can be frequently detected in patients presenting with RTI. Real-time RT-PCR provides a tool for large-scale epidemiological studies to further clarify the role that coronavirus infection plays in RTI in humans.

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