Sensitive and Quantitative Detection of Severe Acute Respiratory Syndrome Coronavirus Infection by Real‐Time Nested Polymerase Chain Reaction | Zendy

Shih Sheng Jiang | Zendy; TsanChi Chen | Zendy; JyhYuan Yang | Zendy; Chao A. Hsiung | Zendy; IhJen Su | Zendy; Yinglan Liu | Zendy; PoCheng Chen | Zendy; JyhLyh Juang | Zendy

Open Access

Sensitive and Quantitative Detection of Severe Acute Respiratory Syndrome Coronavirus Infection by Real‐Time Nested Polymerase Chain Reaction

Author(s) -

Shih Sheng Jiang,

TsanChi Chen,

JyhYuan Yang,

Chao A. Hsiung,

IhJen Su,

Yinglan Liu,

PoCheng Chen,

JyhLyh Juang

Publication year - 2004

Publication title -

clinical infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.44

H-Index - 336

eISSN - 1537-6591

pISSN - 1058-4838

DOI - 10.1086/380841

Subject(s) - real time polymerase chain reaction , nested polymerase chain reaction , polymerase chain reaction , virology , covid-19 , reverse transcriptase , coronavirus , reverse transcription polymerase chain reaction , medicine , biology , gene , pathology , messenger rna , disease , genetics , infectious disease (medical specialty)

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.

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