Cytokine mRNA Expression in Pneumococcal Carriage, Pneumonia, and Sepsis in Young Mice | Zendy

Eduard Ling | Zendy; Galia Feldman | Zendy; Ron Dagan | Zendy; Yaffa MizrachiNebenzahl | Zendy

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Cytokine mRNA Expression in Pneumococcal Carriage, Pneumonia, and Sepsis in Young Mice

Author(s) -

Eduard Ling,

Galia Feldman,

Ron Dagan,

Yaffa MizrachiNebenzahl

Publication year - 2003

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/379371

Subject(s) - tumor necrosis factor alpha , streptococcus pneumoniae , cytokine , immune system , biology , immunology , pneumococcal pneumonia , pneumonia , spleen , serotype , sepsis , microbiology and biotechnology , medicine , antibiotics

We studied cytokine mRNA expression in Streptococcus pneumoniae carriage, pneumonia, and sepsis in 3-week-old, inbred C57BL/6 and BALB/c mice. Mice were inoculated intranasally with S. pneumoniae serotype 6B, 14, or 3. Survival, bacterial load in the nasopharynx (NP) and lungs, and mRNA levels of tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta, interleukin (IL)-10, and IL-12 in the spleen were analyzed. No baseline mRNA expression levels were found, except for TNF-alpha in C57BL/6 mice. Serotype 6B caused NP colonization only, a significant (P<.05) increase in the levels of TNF-alpha, and induction of TGF-beta and IL-12 mRNA. Serotype 14 caused NP and nonlethal lung colonization and induction of TGF-beta, IL-10, and IL-12. Serotype 3 caused NP colonization, pneumonia, 35% mortality, and no alteration in the mRNA expression of tested cytokines. Although activation of the immune system culminated in nonlethal disease, evasion of the immune system was associated with detrimental disease.

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