Pathogen Genotyping in Polyclonal Infections: Application of a Fluorogenic Polymerase–Chain‐Reaction Assay in Malaria | Zendy

Saskia Decuypere | Zendy; Ellen Elinck | Zendy; Chantal Van Overmeir | Zendy; Ambrose Talisuna | Zendy; Umberto D’Alessandro | Zendy; JeanClaude Dujardin | Zendy

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Pathogen Genotyping in Polyclonal Infections: Application of a Fluorogenic Polymerase–Chain‐Reaction Assay in Malaria

Author(s) -

Saskia Decuypere,

Ellen Elinck,

Chantal Van Overmeir,

Ambrose Talisuna,

Umberto D’Alessandro,

JeanClaude Dujardin

Publication year - 2003

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/378521

Subject(s) - genotyping , polymerase chain reaction , pathogen , polyclonal antibodies , malaria , virology , microbiology and biotechnology , biology , plasmodium falciparum , genotype , immunology , antibody , genetics , gene

Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1). how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2). how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples.

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