Leishmania mexicanaandLeishmania major:Attenuation of Wild‐Type Parasites and Vaccination with the Attenuated Lines | Zendy

Hamid Daneshvar | Zendy; Graham H. Coombs | Zendy; Paul Hagan | Zendy; R. S. Phillips | Zendy

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Leishmania mexicanaandLeishmania major:Attenuation of Wild‐Type Parasites and Vaccination with the Attenuated Lines

Author(s) -

Hamid Daneshvar,

Graham H. Coombs,

Paul Hagan,

R. S. Phillips

Publication year - 2003

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/374783

Subject(s) - leishmania mexicana , leishmania , microbiology and biotechnology , attenuated vaccine , in vitro , biology , virology , leishmaniasis , vaccination , leishmania infantum , immunology , parasite hosting , visceral leishmaniasis , virulence , biochemistry , world wide web , computer science , gene

A method for attenuation of Leishmania species by culturing in vitro under gentamicin pressure has been used successfully with Leishmania mexicana, L. major, L. infantum, and L. donovani. The attenuated lines invaded but were unable to survive within bone marrow-derived macrophages in vitro, whereas wild-type parasites survived and multiplied. The attenuated lines of L. mexicana and L. major both failed to induce cutaneous lesions in the majority of BALB/c mice over a minimum 12-week observation period after subcutaneous injection of stationary phase parasites. The attenuated line of L. mexicana retained its properties in gentamicin-free medium over 40 subcultures. The attenuated lines of L. mexicana and L. major both induced significant protection in mice against challenge with wild-type parasites.

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