Analysis of Variation in Tandem Repeats in the Intron of the Major Surface Glycoprotein Expression Site of the Human Form ofPneumocystis carinii | Zendy

Liang Ma | Zendy; Geetha Kutty | Zendy; Qiuyao Jia | Zendy; Hiromi Imamichi | Zendy; Laurence Huang | Zendy; Chiara Atzori | Zendy; Pieter Beckers | Zendy; Gena Groner | Zendy; Charles B. Beard | Zendy; Joseph A. Kovacs | Zendy

Open Access

Analysis of Variation in Tandem Repeats in the Intron of the Major Surface Glycoprotein Expression Site of the Human Form ofPneumocystis carinii

Author(s) -

Liang Ma,

Geetha Kutty,

Qiuyao Jia,

Hiromi Imamichi,

Laurence Huang,

Chiara Atzori,

Pieter Beckers,

Gena Groner,

Charles B. Beard,

Joseph A. Kovacs

Publication year - 2002

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/345721

Subject(s) - pneumocystis carinii , tandem repeat , biology , microsatellite , microbiology and biotechnology , variable number tandem repeat , polymerase chain reaction , gene , genetics , typing , direct repeat , sequence analysis , gel electrophoresis , virology , genotype , allele , genome , human immunodeficiency virus (hiv) , pneumocystis jirovecii

Variation in the tandem repeats in the expression site of the human-derived Pneumocystis carinii major surface glycoprotein gene was characterized by denaturing gel electrophoresis. The number of repeats in 147 isolates ranged from 2 to 6, with 2, 3, and 4 repeats being the most common. Sequence analysis identified 3 types of repeat units that differed by 1 nucleotide, which suggests a hierarchy of evolution of human-derived P. carinii. Examination of sequential samples obtained from 6 patients at an interval of 10-90 days showed an identical repeat pattern in each patient. However, in 2 of 4 patients with 2-3 different samples obtained within 4 days, different repeat patterns were observed among the samples. Quantifying the number of repeats by denaturing gel electrophoresis is a simple and rapid-typing method that can be used alone or in combination with other methods to study the epidemiology of P. carinii.

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