HelicobacterSpecies Ribosomal DNA Recovered from the Liver Tissue of Chinese Patients with Primary Hepatocellular Carcinoma | Zendy

Xuegong Fan | Zendy; XiaoNing Peng | Zendy; Yan Huang | Zendy; Javed Yakoob | Zendy; Zhiming Wang | Zendy; YongPing Chen | Zendy

Open Access

HelicobacterSpecies Ribosomal DNA Recovered from the Liver Tissue of Chinese Patients with Primary Hepatocellular Carcinoma

Author(s) -

Xuegong Fan,

XiaoNing Peng,

Yan Huang,

Javed Yakoob,

Zhiming Wang,

YongPing Chen

Publication year - 2002

Publication title -

clinical infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.44

H-Index - 336

eISSN - 1537-6591

pISSN - 1058-4838

DOI - 10.1086/344770

Subject(s) - polymerase chain reaction , ribosomal dna , 16s ribosomal rna , hepatocellular carcinoma , helicobacter pylori , ribosomal rna , helicobacter , biology , medicine , microbiology and biotechnology , bacteria , pathology , gene , genetics , phylogenetics

Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragments from positive PCR samples were directly sequenced. By sequence comparison, all were found to be 99% identical to the 16S rRNA of Helicobacter pylori.

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