Open AccessMultilaboratory Comparison of Anaerobe Susceptibility Results Using 3 Different Agar Media
Author(s) -
Darcie E. Roe,
S M Finegold,
Diane M. Citron,
Ellie J. C. Goldstein,
Hannah M. Wexler,
Jon E. Rosenblatt,
Mike E. Cox,
Stephen G. Jenkins,
D. W. Hecht
Publication year - 2002
Publication title -
clinical infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1086/341919
Subject(s) - ceftizoxime , microbiology and biotechnology , cefoxitin , piperacillin , antibiotics , trovafloxacin , medicine , agar dilution , minimum inhibitory concentration , anaerobic bacteria , agar , clindamycin , biology , antibacterial agent , bacteria , cefotaxime , staphylococcus aureus , genetics , pseudomonas aeruginosa
A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of >0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.
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