Open AccessMultilaboratory Comparison of Growth Characteristics for Anaerobes, Using 5 Different Agar Media
Author(s) -
Darcie E. Roe,
S M Finegold,
Diane M. Citron,
E. J. C. Goldstein,
Hannah M. Wexler,
Jon E. Rosenblatt,
Mike E. Cox,
Stephen G. Jenkins,
D. W. Hecht
Publication year - 2002
Publication title -
clinical infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1086/341918
Subject(s) - fastidious organism , anaerobic exercise , microbiology and biotechnology , whole blood , macconkey agar , brucella , agar plate , incubation , agar , bacterial growth , biology , medicine , food science , bacteria , veterinary medicine , physiology , brucellosis , biochemistry , genetics
A multilaboratory study compared the growth of 30 fastidious anaerobes, using 5 different agar media: Wilkins-Chalgren (WC), WC with either whole or laked sheep blood, and Brucella supplemented with vitamin K(1) and hemin and either laked or whole sheep blood. The media were compared for quality and quantity of growth. Experiments were conducted either entirely in an anaerobic chamber or inoculated in ambient air with anaerobic incubation. The results showed that (1) any medium plus whole or laked blood was better than unsupplemented WC, (2) whole blood and laked blood additives gave similar results, (3) supplemented Brucella with whole or laked blood was superior to WC and WC with whole or laked blood, and (4) anaerobic and aerobic inoculation with anaerobic incubation gave similar results. Brucella agar supplemented with whole or laked blood supports the growth of fastidious anaerobic species better than the WC agars do.
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