Open AccessDemonstration of a Cell‐Mediated Immune Response in Melioidosis
Author(s) -
Natkunam Ketheesan,
Jodie L. Barnes,
Glen C. Ulett,
Helen J. VanGessel,
Robert Norton,
Robert G. Hirst,
Justin T. LaBrooy
Publication year - 2002
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/341222
Subject(s) - burkholderia pseudomallei , melioidosis , immune system , immunology , antigen , cd8 , biology , lymphocyte , acquired immune system , t cell , peripheral blood mononuclear cell , t lymphocyte , antibody , cellular immunity , microbiology and biotechnology , bacteria , in vitro , biochemistry , genetics
Melioidosis is a bacterial infection caused by Burkholderia pseudomallei. The aim of this study was to determine whether a cell-mediated adaptive immune response against B. pseudomallei developed in patients who had recovered from melioidosis. Lymphocyte proliferation assays were done on peripheral blood mononuclear cells from patients (n=13) and control subjects (n=10) to determine the lymphocyte response to B. pseudomallei antigens. Production of interferon-gamma and interleukin-10 was also determined. Activation of T cell subsets was assessed by fluorescence-activated cell sorter analysis, using antibodies to CD4, CD8, and CD69 antigens. Lymphocyte proliferation and interferon-gamma production in response to B. pseudomallei antigens were significantly higher (P<.001 for both) in patients than in control subjects. There was also an increase in the percentage of activated CD4+ (P<.004) and activated CD8+ T cells (P<.035) in cell cultures from patients. The development of such a cell-mediated immune response in patients may be essential for their survival.
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