Effect of Macrolide Antibiotics on Human Endothelial Cells Activated byChlamydia pneumoniaeInfection and Tumor Necrosis Factor–α | Zendy

Silvia M. Uriarte | Zendy; Robert E. Molestina | Zendy; Richard D. Miller | Zendy; Jorge Guillermo Bernabó | Zendy; Alicia Farinati | Zendy; Kumiko Eiguchi | Zendy; Julio A. Ramírez | Zendy; James T. Summersgill | Zendy

Open Access

Effect of Macrolide Antibiotics on Human Endothelial Cells Activated byChlamydia pneumoniaeInfection and Tumor Necrosis Factor–α

Author(s) -

Silvia M. Uriarte,

Robert E. Molestina,

Richard D. Miller,

Jorge Guillermo Bernabó,

Alicia Farinati,

Kumiko Eiguchi,

Julio A. Ramírez,

James T. Summersgill

Publication year - 2002

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/340575

Subject(s) - roxithromycin , tumor necrosis factor alpha , monocyte , azithromycin , clarithromycin , microbiology and biotechnology , umbilical vein , antibiotics , protein synthesis inhibitor , chlamydia , interleukin 8 , macrolide antibiotics , immunology , antibacterial agent , cytokine , biology , medicine , in vitro , erythromycin , biochemistry

This study investigated the potential anti-inflammatory activity of 3 macrolide antibiotics, clarithromycin, roxithromycin, and azithromycin, in an in vitro model of transendothelial migration (TEM). Human umbilical vein endothelial cells (HUVECs) were seeded in Transwell inserts, treated with serial dilutions of the antibiotics, and infected with Chlamydia pneumoniae or stimulated with tumor necrosis factor (TNF)-alpha. In HUVECs infected with C. pneumoniae or stimulated with TNF-alpha, both azithromycin and roxithromycin caused significant decreases in neutrophil and monocyte TEM, compared with antibiotic-free controls. Clarithromycin had no detectable effect in either group. Azithromycin caused significant decreases in interleukin (IL)-8 and monocyte chemotactic protein (MCP)-1, whereas roxithromycin significantly decreased IL-8. This study indicates heterogeneity in the anti-inflammatory activity of these antibiotics. Mechanisms of monocyte and neutrophil TEM inhibition by azithromycin and roxithromycin are unclear but may be partially due to inhibition of IL-8 and MCP-1 production.

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