Inhibition of Vancomycin‐Resistant Enterococci by an In Vitro Continuous‐Flow Competitive Exclusion Culture Containing Human Stool Flora | Zendy

Curtis J. Donskey | Zendy; Michael E. Hume | Zendy; Todd R. Callaway | Zendy; Sarbani M. Das | Zendy; Claudia Hoyen | Zendy; Louis B. Rice | Zendy

Open Access

Inhibition of Vancomycin‐Resistant Enterococci by an In Vitro Continuous‐Flow Competitive Exclusion Culture Containing Human Stool Flora

Author(s) -

Curtis J. Donskey,

Michael E. Hume,

Todd R. Callaway,

Sarbani M. Das,

Claudia Hoyen,

Louis B. Rice

Publication year - 2001

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/324533

Subject(s) - microbiology and biotechnology , enterococcus faecium , flora (microbiology) , enterococcus , in vitro , competitive exclusion , biology , vancomycin , feces , bacteria , antibiotics , staphylococcus aureus , biochemistry , ecology , competition (biology) , genetics

An in vitro anaerobic continuous-flow competitive exclusion (CFCE) culture model was used to study the ability of human stool flora to inhibit the growth of vancomycin-resistant (VR) enterococci (VRE). The CFCE culture was established from a stool sample obtained from a healthy adult. When 10(3) or 10(6) cfu/mL of VR Enterococcus faecium were added to the CFCE culture, the VRE were eliminated within 6 or 9 days, respectively. When 10(7) cfu/mL of the CFCE culture was added to a continuous-flow culture that contained 10(7) cfu/mL of VRE, the density of VRE was reduced but not eliminated. These data support the hypothesis that the indigenous intestinal flora inhibit growth of VRE and suggest that CFCE cultures may be a useful means to study interactions between the indigenous flora and VRE.

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