Neutralization of Human Papillomavirus Type 11 (HPV‐11) by Serum from Women Vaccinated with Yeast‐Derived HPV‐11 L1 Virus‐like Particles: Correlation with Competitive Radioimmunoassay Titer | Zendy

Darron R. Brown | Zendy; Janine T. Bryan | Zendy; Jill M. Schroeder | Zendy; Tiffany S. Robinson | Zendy; Kenneth H. Fife | Zendy; Cosette M. Wheeler | Zendy; Eliav Barr | Zendy; Paula Smith | Zendy; Lisa M. Chiacchierini | Zendy; ANTHONY C. DiCELLO | Zendy; Kathrin U. Jansen | Zendy

Open Access

Neutralization of Human Papillomavirus Type 11 (HPV‐11) by Serum from Women Vaccinated with Yeast‐Derived HPV‐11 L1 Virus‐like Particles: Correlation with Competitive Radioimmunoassay Titer

Author(s) -

Darron R. Brown,

Janine T. Bryan,

Jill M. Schroeder,

Tiffany S. Robinson,

Kenneth H. Fife,

Cosette M. Wheeler,

Eliav Barr,

Paula Smith,

Lisa M. Chiacchierini,

ANTHONY C. DiCELLO,

Kathrin U. Jansen

Publication year - 2001

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/323645

Subject(s) - neutralization , titer , immunogenicity , virology , neutralizing antibody , radioimmunoassay , antibody , medicine , virus , immunology , biology

Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1 VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive radioimmunoassay (cRIA) and for neutralization by use of the athymic mouse xenograft system. Analysis of serum from 104 subjects showed a dose response in HPV-11 cRIA titers and neutralization. Overall, 68 (82.9%) of 82 postimmunization serum specimens from VLP recipients were 100% neutralizing when used in the assay at a 1:50 dilution. Of 69 serum specimens, 63 (91.3%) with cRIA titers >200 milliMerck units per milliliter were neutralizing. Immunization with HPV VLPs elicits a vigorous serum immune response in a high percentage of women. The HPV-11 cRIA titer appears to be a surrogate marker for neutralization.

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