Development and Characterization of a Molecular Viability Assay forPneumocystis cariniif sphominis | Zendy

Nancy Maher | Zendy; Sten H. Vermund | Zendy; David A. Welsh | Zendy; H. Kenneth Dillon | Zendy; Abeer Awooda | Zendy; Thomas R. Unnasch | Zendy

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Development and Characterization of a Molecular Viability Assay forPneumocystis cariniif sphominis

Author(s) -

Nancy Maher,

Sten H. Vermund,

David A. Welsh,

H. Kenneth Dillon,

Abeer Awooda,

Thomas R. Unnasch

Publication year - 2001

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/320738

Subject(s) - pneumocystis carinii , pneumonia , virology , biology , microbiology and biotechnology , transmission (telecommunications) , aids related opportunistic infections , human immunodeficiency virus (hiv) , sida , viral disease , medicine , pneumocystis jirovecii , electrical engineering , engineering

Pneumocystis carinii pneumonia (PCP) remains the most common opportunistic infection among human immunodeficiency virus-infected persons. Despite this, little is known concerning the transmission dynamics of this infection. In the absence of a reliable method to isolate and culture P. carinii from environmental samples, it has not been possible to assess the importance of person-to-person transmission in the epidemiology of PCP. A molecular viability assay was developed for the human form of P. carinii (P. carinii f sp hominis) that is applicable to both clinical specimens and environmental samples. This assay will enable the evaluation of the spread and persistence of viable human P. carinii in the environment.

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