A Whole Blood Bactericidal Assay for Tuberculosis | Zendy

Robert S. Wallis | Zendy; Moisés Palaci | Zendy; Solange Alves Vinhas | Zendy; Amy G. Hise | Zendy; Fabiola Correa Ribeiro | Zendy; Katherine Landen | Zendy; SeonHee Cheon | Zendy; HoYeon Song | Zendy; Manijeh Phillips | Zendy; Reynaldo Dietze | Zendy; Jerrold J. Ellner | Zendy

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A Whole Blood Bactericidal Assay for Tuberculosis

Author(s) -

Robert S. Wallis,

Moisés Palaci,

Solange Alves Vinhas,

Amy G. Hise,

Fabiola Correa Ribeiro,

Katherine Landen,

SeonHee Cheon,

HoYeon Song,

Manijeh Phillips,

Reynaldo Dietze,

Jerrold J. Ellner

Publication year - 2001

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/319679

Subject(s) - pyrazinamide , ethambutol , isoniazid , levofloxacin , microbiology and biotechnology , tuberculosis , medicine , ofloxacin , mycobacterium tuberculosis , pharmacology , amoxicillin , antibacterial agent , antibiotics , whole blood , biology , immunology , ciprofloxacin , pathology

The bactericidal activity of orally administered antituberculosis (anti-TB) drugs was determined in a whole blood culture model of intracellular infection in which microbial killing reflects the combined effects of drug and immune mechanisms. Rifampin (Rif) was the most active compound studied and reduced the number of viable bacilli by >4 logs. Isoniazid (INH), 2 quinolones, and pyrazinamide (PZA) showed intermediate levels of activity. Ethambutol exerted only a bacteristatic effect; amoxicillin/clavulanate was inactive. The combination of INH-Rif-PZA showed strong activity against 11 drug-sensitive isolates (mean, -3.8 log) but no activity against 12 multidrug-resistant (MDR) strains. The combination of levofloxacin-PZA-ethambutol had intermediate bactericidal activity against MDR isolates (mean, -1.2 log) but failed to equal that of INH-Rif-PZA against sensitive isolates (P<.001). The whole blood BACTEC method (Becton Dickinson) may be useful for the early clinical evaluation of new anti-TB drugs and in the management of individual patients.

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