Human Macrophage Tumor Necrosis Factor (TNF)–α Production Induced byTrypanosoma brucei gambienseand the Role of TNF‐α in Parasite Control | Zendy

Sylvie Daulouède | Zendy; Bernard Bouteille | Zendy; Daniel Moynet | Zendy; Patrick De Baetselier | Zendy; Pierrette Courtois | Zendy; J. L. Lemesre | Zendy; Alain Buguet | Zendy; Raymond Cespuglio | Zendy; Philippe Vincendeau | Zendy

Open Access

Human Macrophage Tumor Necrosis Factor (TNF)–α Production Induced byTrypanosoma brucei gambienseand the Role of TNF‐α in Parasite Control

Author(s) -

Sylvie Daulouède,

Bernard Bouteille,

Daniel Moynet,

Patrick De Baetselier,

Pierrette Courtois,

J. L. Lemesre,

Alain Buguet,

Raymond Cespuglio,

Philippe Vincendeau

Publication year - 2001

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/319257

Subject(s) - tumor necrosis factor alpha , biology , lytic cycle , trypanosomiasis , immunology , macrophage , african trypanosomiasis , cytokine , parasite hosting , virology , in vitro , trypanosoma , microbiology and biotechnology , virus , biochemistry , world wide web , computer science

Trypanosoma brucei gambiense, a causative agent of sleeping sickness, induced a dose-dependent production of tumor necrosis factor (TNF)-alpha by human macrophages in vitro. TNF-alpha was also induced in the Mono Mac 6 cell line, which indicates a direct effect of parasite components on macrophages. Parasite-soluble factors were also potent inducers of TNF-alpha. The addition of anti-TNF-alpha to cocultures of macrophages and parasites increased the number of trypanosomes and their life span, whereas irrelevant antibodies had no effect. TNF-alpha may have a direct role (i.e., direct trypanolytic activity) and/or an indirect one, such as TNF-alpha-mediated induction of cytotoxic molecules. A direct dose-dependent lytic effect of TNF-alpha on purified parasites was observed. This lytic effect was inhibited by anti-TNF-alpha. These data suggest that, as in experimental trypanosomiasis, TNF-alpha is involved in parasite growth control in human African trypanosomiasis.

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