Open AccessProduction of Interleukin‐18 in Human Tuberculosis
Author(s) -
Ramakrishna Vankayalapati,
Benjamin Wizel,
Stephen E. Weis,
Buka Samten,
William M. Girard,
Peter F. Barnes
Publication year - 2000
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/315656
Subject(s) - tuberculosis , immunology , peripheral blood mononuclear cell , mycobacterium tuberculosis , medicine , interferon gamma , tuberculin , cytokine , nontuberculous mycobacteria , mycobacterium , biology , pathology , in vitro , biochemistry
To investigate the role of interleukin (IL)-18 in human tuberculosis, IL-18 production was evaluated in blood and at the site of disease in patients with tuberculosis. Mycobacterium tuberculosis-stimulated peripheral blood mononuclear cells (PBMC) from tuberculosis patients secreted less IL-18 and interferon-gamma (IFN-gamma) than did PBMC from healthy persons reactive to tuberculin. M. tuberculosis-induced IFN-gamma production was inhibited by anti-IL-18 and enhanced by recombinant IL-18. Alveolar macrophages secreted IL-18 in response to M. tuberculosis, and IL-18 and IFN-gamma concentrations were higher in pleural fluid of patients with tuberculosis than in pleural fluid of patients with nontuberculous diseases. These findings demonstrate that IL-18 production by PBMC correlates with IFN-gamma production and effective immunity to tuberculosis, suggesting that IL-18 contributes to a protective type 1 cytokine response in persons with mycobacterial infection.
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