Open AccessDiagnosis of Human Caliciviruses by Use of Enzyme Immunoassays
Author(s) -
Xi Jiang,
Nouansy Wilton,
Wenyu Zhong,
Tibor Farkas,
Pengwei Huang,
Elizabeth Barrett,
M. L. Guerrero,
Guillermo M. RuizPalacios,
K. Y. Green,
J. Green,
A. Hale,
M K Estes,
Larry K. Pickering,
David O. Matson
Publication year - 2000
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/315577
Subject(s) - capsid , norwalk virus , antigen , virology , calicivirus , virus , caliciviridae , virus like particle , biology , antibody , recombinant dna , gene , viral disease , immunology , genetics , norovirus
The application of molecular technologies, such as the expression of viral proteins in baculovirus, has provided a powerful approach to the diagnosis of human calicivirus (HuCV) infections. The baculovirus-expressed HuCV capsid protein self-assembles into virus-like particles, providing excellent reagents for immunologic assays, such as enzyme immunoassays (EIAs). Following the expression of the capsid protein of Norwalk virus, the capsid proteins of 8 other HuCV strains have been expressed in baculovirus. The unlimited supply of baculovirus-produced reagents for HuCVs allows these EIAs to be applied in large-scale clinical and epidemiological studies. Both the antigen and antibody-detection EIAs are highly sensitive. The antigen-detection EIAs are highly specific, but the antibody-detection EIAs are more broadly reactive. This article reviews baculovirus expression techniques used to produce HuCV capsid antigens, development of EIAs using these antigens, and application of these EIAs in studies of HuCV infection and illness.
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