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Candida endophthalmitis is a serious infection secondary to hematogenous dissemination or direct inoculation of the organisms following trauma or eye surgery. The diagnosis is based on the characteristic findings in the infected eye and on culture of vitreous samples. Unfortunately, the yield of vitreous cultures is limited. The use of a Candida albicans species-specific polymerase chain reaction (PCR) assay in the diagnosis of Candida endophthalmitis is reported herein. Four patients with suspected fungal endophthalmitis underwent vitrectomy for diagnostic and therapeutic purposes. In 2 of the 4, vitreous cultures were negative. However, characteristic PCR products were generated in all 4 patient specimens, enabling the rapid diagnosis of Candida endophthalmitis in all 4. Clinical response was observed in all cases. These results demonstrate the utility of PCR-mediated detection of C. albicans in vitreous samples.

the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases

Fungal Endophthalmitis Diagnosis by Detection of<i>Candida albicans</i>DNA in Intraocular Fluid by Use of a Species‐Specific Polymerase Chain Reaction Assay

Fungal Endophthalmitis Diagnosis by Detection ofCandida albicansDNA in Intraocular Fluid by Use of a Species‐Specific Polymerase Chain Reaction Assay