Open AccessRecombinant Human Interleukin‐11 Has Anti‐inflammatory Actions Yet Does Not Exacerbate SystemicListeriaInfection
Author(s) -
Steven M. Opal,
James C. Keith,
John E. Palardy,
Nicolas A. Parejo
Publication year - 2000
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/315247
Subject(s) - listeria monocytogenes , tumor necrosis factor alpha , cytokine , monoclonal antibody , interleukin , listeria infection , recombinant dna , immunology , listeria , antibody , biology , microbiology and biotechnology , medicine , bacteria , biochemistry , genetics , gene
To determine whether recombinant human (rh) interleukin (IL)-11 disrupts the clearance of microbial pathogens, mice were challenged with Listeria monocytogenes after receiving high-dose rhIL-11, anti-tumor necrosis factor (TNF) monoclonal antibody (MAb), anti-IL-11 MAb, or saline control. The LD50 was not affected by rhIL-11 but was 10-fold lower in the anti-TNF MAb group (P<.001). Plasma IL-6, IL-1beta, and TNF-alpha levels were not different between rhIL-11-treated animals and the control group; however, interferon-gamma levels were significantly reduced by IL-11 treatment (2477 vs. 0 pg/mL, P<.01). Compared with the control group, the quantitative level of L. monocytogenes in hepatic and splenic tissue was unchanged by rhIL-11 but was significantly increased by TNF or IL-11 inhibition. The results indicate that IL-11 down-regulates cytokine production but does not exacerbate systemic infection in the murine Listeria infection model.
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