Open AccessMultiepitope Synthetic Peptide and Recombinant Protein for the Detection of Antibodies toTrypanosoma cruziin Patients with Treated or Untreated Chagas' Disease
Author(s) -
Raymond L. Houghton,
Darin R. Benson,
Lisa D. Reynolds,
Patricia D. McNeill,
Paul R. Sleath,
Michael J. Lodes,
Yasir A. W. Skeiky,
Roberto Badaró,
Antoniana U. Krettli,
Steven G. Reed
Publication year - 2000
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/315165
Subject(s) - trypanosoma cruzi , chagas disease , antibody , virology , recombinant dna , biology , epitope , antigen , tetrapeptide , microbiology and biotechnology , immunology , peptide , parasite hosting , biochemistry , world wide web , computer science , gene
A tetrapeptide and a recombinant protein, each representing 4 immunodominant epitopes of Trypanosoma cruzi, were tested by use of ELISA for the detection of serum antibodies. Sera from individuals with Chagas' disease, including persons untreated and successfully or unsuccessfully treated, were tested. These assays detected antibody in 100% of the parasitemias. The antibody reactivity decreased based on the success of treatment. Higher sensitivity was observed for tetrapeptide/recombinant protein assays than for lysate-based ELISA, and specificity was improved, particularly with Leishmania sera. The results indicate that multiepitope antigens provide a more sensitive and specific alternative to lysate for detection of anti-T. cruzi antibodies, as required for developing blood screening assays.
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