Phenotypic Analysis of Human Immunodeficiency Virus (HIV) Type 1 Cell‐Mediated Immune Responses after Treatment with an HIV‐1 Immunogen | Zendy

Ronald B. Moss | Zendy; Mark R. Wallace | Zendy; Wieslawa Giermakowska | Zendy; Erin Webb | Zendy; Jay R. Savary | Zendy; Carolyn ChamberlinBrandt | Zendy; Georgia Theofan | Zendy; Roy Musil | Zendy; Steven P. Richieri | Zendy; Fred C. Jensen | Zendy; Dennis J. Carlo | Zendy

Open Access

Phenotypic Analysis of Human Immunodeficiency Virus (HIV) Type 1 Cell‐Mediated Immune Responses after Treatment with an HIV‐1 Immunogen

Author(s) -

Ronald B. Moss,

Mark R. Wallace,

Wieslawa Giermakowska,

Erin Webb,

Jay R. Savary,

Carolyn ChamberlinBrandt,

Georgia Theofan,

Roy Musil,

Steven P. Richieri,

Fred C. Jensen,

Dennis J. Carlo

Publication year - 1999

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/314924

Subject(s) - immunogen , immune system , immunology , cd8 , hiv antigens , antigen , virology , lymphocyte , biology , t lymphocyte , t cell , virus , flow cytometry , antibody , viral disease , monoclonal antibody

It was hypothesized that immune recognition could be stimulated with combined immune-based and potent antiviral drug therapies. This study examined human immunodeficiency virus type 1 (HIV-1)-specific lymphocyte proliferation before and after treatment with an inactivated HIV-1 immunogen in 15 chronically infected HIV-1 seropositive subjects. Lymphocyte proliferation to the immunizing antigen (gp120-depleted HIV-1; P<.001), purified native p24 (P<.001), and recombinant p24 (P<.05) increased after treatment with the HIV-specific immune-based therapy. By HIV-1 antigen-specific flow cytometry, T helper CD4 lymphocytes, CD8 lymphocytes, and NK cells (all P<.001) were the predominant cell types proliferating in vitro after treatment. Additional phenotyping of proliferating cells revealed predominantly CD4 and CD8 memory (both P<.001) phenotypes. This study supports the concept that in vitro lymphocyte proliferation to HIV-1 antigens, augmented after treatment with an inactivated HIV-1 immunogen, involves primarily CD4 and CD8 cell memory immune responses.

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