Open AccessType‐Specific Opsonophagocytosis of Group A Streptococcus by Use of a Rapid Chemiluminescence Assay
Author(s) -
Alberto VillaseñorS,
Amy E. Bryant,
Dennis L. Stevens
Publication year - 1999
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/314719
Subject(s) - opsonin , heterologous , chemiluminescence , microbiology and biotechnology , chemistry , streptococcus , phagocyte , streptococcus pyogenes , antibody , antibody opsonization , immunology , phagocytosis , bacteria , biology , chromatography , biochemistry , genetics , gene , staphylococcus aureus
A whole-blood chemiluminescence (CL) assay was developed to determine the presence of type-specific opsonic antibodies against group A streptococcus (GAS). Convalescent sera with high bactericidal activities against an M-1 serotype were used to opsonize different M-types of GAS. CL responses were monitored for 20 min, and results were expressed as integral counts/minute per phagocyte. CL responses of phagocytes incubated with M-1 GAS opsonized with homologous (M-1) serum were significantly higher than responses of phagocytes incubated with heterologous (M-3) GAS. Adsorption of convalescent serum against the homologous, but not the heterologous, strain markedly reduced the CL response, demonstrating type specificity. The CL assay showed a high correlation with the indirect bactericidal test (r=0.90). In conclusion, this CL assay is a rapid, highly sensitive, specific, and reproducible method for quantifying type-specific opsonic antibodies against GAS and will be a useful tool for future clinical, basic science, and epidemiological studies.
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