Recombinant Influenza A Virus Vaccines for the Pathogenic Human A/Hong Kong/97 (H5N1) Viruses | Zendy

Shengqiang Li | Zendy; Chongguang Liu | Zendy; Alexander Klimov | Zendy; Kanta Subbarao | Zendy; Michael L. Perdue | Zendy; Delia Mo | Zendy; Yaying Ji | Zendy; Leslie W. Woods | Zendy; Sharon K. Hietala | Zendy; Martin L. Bryant | Zendy

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Recombinant Influenza A Virus Vaccines for the Pathogenic Human A/Hong Kong/97 (H5N1) Viruses

Author(s) -

Shengqiang Li,

Chongguang Liu,

Alexander Klimov,

Kanta Subbarao,

Michael L. Perdue,

Delia Mo,

Yaying Ji,

Leslie W. Woods,

Sharon K. Hietala,

Martin L. Bryant

Publication year - 1999

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/314713

Subject(s) - virology , influenza a virus subtype h5n1 , neuraminidase , biology , virus , reassortment , hemagglutinin (influenza) , recombinant dna , h5n1 genetic structure , attenuated vaccine , recombinant virus , orthomyxoviridae , influenza a virus , inactivated vaccine , gene , microbiology and biotechnology , virulence , genetics , medicine , infectious disease (medical specialty) , disease , covid-19 , pathology

Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each H5N1 isolate was modified to resemble that of "low-pathogenic" avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5-9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.

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