Open AccessEnhancement of Pulmonary Clearance ofMoraxella (Branhamella) catarrhalisfollowing Immunization with Outer Membrane Protein CD in a Mouse Model
Author(s) -
Timothy F. Murphy,
Jennelle M. Kyd,
Anna John,
Charmaine Kirkham,
Allan W. Cripps
Publication year - 1998
Publication title -
the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/314501
Subject(s) - moraxella catarrhalis , moraxella (branhamella) catarrhalis , immunization , immunology , moraxella , respiratory tract , microbiology and biotechnology , immune system , bronchoalveolar lavage , antigen , antibody , biology , medicine , lung , respiratory system , streptococcus pneumoniae , bacteria , genetics , anatomy , antibiotics
Moraxella (Branhamella) catarrhalis is an important human respiratory tract pathogen. Outer membrane protein (OMP) CD is highly conserved among strains and has characteristics that indicate it may be an effective vaccine antigen. This study investigated the effect of immunization with OMP CD on pulmonary clearance following intratracheal challenge of mice with M. catarrhalis. Two routes of immunization were studied: mucosal immunization (intra-Peyer's patch followed by intratracheal boost) and intramuscular immunization with OMP CD. Both resulted in enhanced pulmonary clearance of M. catarrhalis compared with sham-immunized controls. Immunization with OMP CD induced specific antibodies in serum and bronchoalveolar lavage fluid and induced a specific lymphocyte proliferative response in T cells from mesenteric lymph nodes from mice mucosally immunized with OMP CD. On the basis of these results, OMP CD should undergo continued testing to determine whether it will induce a protective immune response in humans.