Open AccessPriming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes.
Author(s) -
R B Johnston,
Donna A. Chadwick,
Z. A. Cohn
Publication year - 1981
Publication title -
the journal of experimental medicine/the journal of experimental medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.483
H-Index - 448
eISSN - 1540-9538
pISSN - 0022-1007
DOI - 10.1084/jem.153.6.1678
Subject(s) - proteases , biochemistry , pronase , superoxide , proteolytic enzymes , respiratory burst , elastase , oxidative phosphorylation , trypsin , zymosan , biology , phagocytosis , chemistry , enzyme , microbiology and biotechnology , in vitro
Preincubation for 10-30 min with trypsin, pronase, chymotrypsin, or papain primed macrophages to undergo a twofold to sixfold increase in oxidative metabolism, measured as release of superoxide anion or hydrogen peroxide, during stimulation by phorbol myristate acetate or ingestion of Candida parapsilosis. Preincubation of macrophages with inactivated proteases, nonenzyme proteins, or neuraminidase did not affect their oxidase response. Exposure of macrophages to proteases generated at sites of inflammation could prime these cells for a more effective oxidase response to phagocytosis or for greater tissue damage from release of toxic oxygen metabolites.