STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES | Zendy

Richard M. Condie | Zendy; Antonio Howell | Zendy; Robert A. Good | Zendy

Open Access

STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND EMBEDDING TECHNIQUES

Author(s) -

Richard M. Condie,

Antonio Howell,

Robert A. Good

Publication year - 1961

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.9.2.429

Subject(s) - myelin sheath , myelin , dehydration , biology , fixation (population genetics) , ultrastructure , electron microscope , anatomy , microbiology and biotechnology , biochemistry , neuroscience , central nervous system , physics , gene , optics

Currently accepted methods of tissue preparation for electron microscopy result in alterations of myelinated nerve fibers. In an attempt to minimize distortion of myelin, various fixation techniques, dehydration schedules, and embedding methods have been evaluated. It was found that the major damage to myelinated nerves occurs in the embedding procedure. A technique for embedding nerve tissue using the polyester Vestopal W is described which was found to result in improved preservation of myelin.

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