Preservation of Integrity of Rat Tissues for Cytochemical Staining Purposes | Zendy

S. J. Holt | Zendy; Eluned E. Hobbiger | Zendy; G. L. S. Pawan | Zendy

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Preservation of Integrity of Rat Tissues for Cytochemical Staining Purposes

Author(s) -

S. J. Holt,

Eluned E. Hobbiger,

G. L. S. Pawan

Publication year - 1960

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.7.2.383

Subject(s) - biology , staining , microbiology and biotechnology , genetics

The use of sucrose solutions as suspending media for preparation of tissue homogenates and subsequent centrifugal fractionation has become a widely accepted procedure. Recently, it has been claimed that addition of colloids to the medium results in better preservation of the morphological and enzymic integrity of the isolated fractions (4, 8, 15). Such media also have valuable applications during the preparation of tissues for eytochemical staining procedures. Brief mention has already been made elsewhere

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