An Electron Microscopic Investigation into the Effect of EDTA on Plant Cell Wall | Zendy

Shimon Klein | Zendy; B. Z. Ginzburg | Zendy

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An Electron Microscopic Investigation into the Effect of EDTA on Plant Cell Wall

Author(s) -

Shimon Klein,

B. Z. Ginzburg

Publication year - 1960

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.7.2.335

Subject(s) - uranyl acetate , uranyl , cell wall , cellulose , electron microscope , fixation (population genetics) , biology , biophysics , chelation , cellulose acetate , ion , ultrastructure , nuclear chemistry , biochemistry , chemical engineering , botany , materials science , chemistry , metallurgy , organic chemistry , physics , gene , optics , engineering

To study the effect of EDTA on cell wall structure and the reversal of this effect by uranyl ion, thin sections of pea root tips were examined in the electron microscope. EDTA is known to facilitate separation of the cells in root tips. When sections of fixed and embedded EDTA-treated roots are floated on a uranyl-acetate solution, a loose network is revealed that would seem to be cellulose. Incorporation of uranyl into the roots, if it occurs prior to fixation, brings about recementation of the cells. After such treatment, a marginal darker area and a median brighter one can be observed in the wall, and the whole structure appears more compact again. Comparison of the results of the various treatments suggests that cellulose-cementing material is dispersed throughout the entire wall, and that its distribution parallels that of cellulose.

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