Open AccessThe Isolation of Nucleoli from Ungerminated Pea Embryos
Author(s) -
F. B. Johnston,
G. Setterfield,
Herbert Stern
Publication year - 1959
Publication title -
the journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.414
H-Index - 380
eISSN - 1540-8140
pISSN - 0021-9525
DOI - 10.1083/jcb.6.1.53
Subject(s) - nucleolus , biology , embryo , isolation (microbiology) , microbiology and biotechnology , nucleus , bioinformatics
A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.
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