Cell Fractionation of Anterior Pituitary Glands from Beef and Pig | Zendy

Frank S. LaBella | Zendy; Jennifer Brown | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cell Fractionation of Anterior Pituitary Glands from Beef and Pig

Author(s) -

Frank S. LaBella,

Jennifer Brown

Publication year - 1959

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.5.1.17

Subject(s) - biology , differential centrifugation , microsome , biochemistry , cell fractionation , acid phosphatase , cytoplasm , mitochondrion , centrifugation , alkaline phosphatase , microbiology and biotechnology , enzyme

Fresh anterior pituitary glands from beef and pig were separated by differential centrifugation into subcellular fractions. Nuclei and debris were obtained at 700 g for 15 minutes, secretory granules at 7000 g for 20 minutes, mitochondria at 34,000 g for 15 minutes, and microsomes at 78,000 g for 3 hours. Electron micrographs were taken of the individual fractions. Each fraction was analyzed for nitrogen, pentosenucleic acid (PNA), and phospholipide. Beef and pig anterior lobes were quite similar in their intracellular composition as seen in the subcellular fractions. Succinic dehydrogenase was localized in mitochondria, while alkaline phosphatase was concentrated in the microsomes. A proteinase with pH optimum at 8.2 was exclusively localized. in microsomal and supernatant fractions. Acid phosphatase, acid ribonuclease, and acid proteinase were distributed among the subcellular fractions in another pattern, indicating the presence of a particle type distinct from mitochondria and microsomes. The distribution of cytoplasmic PNA paralleled that of alkaline phosphatase.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research