The Effect of HCl Hydrolysis on the Retention of Thymidine in DNA | Zendy

Rachel E. Savage | Zendy; W. Plaut | Zendy

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The Effect of HCl Hydrolysis on the Retention of Thymidine in DNA

Author(s) -

Rachel E. Savage,

W. Plaut

Publication year - 1958

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.4.6.701

Subject(s) - feulgen stain , thymidine , hydrolysis , biology , dna , hydrochloric acid , biochemistry , staining , acid hydrolysis , chromatography , chemistry , genetics , organic chemistry

Allium roots grown in C(14)-thymidine and H(3)-thymidine media were treated with N hydrochloric acid at 60 degrees C. as in standard Feulgen hydrolysis. The retention of the radioactive thymidine in DNA as a function of hydrolysis time was studied autoradiographically. No significant loss of label was detected until hydrolysis was extended beyond the optimal time for Feulgen staining. The data are consistent with the assumption that there is no significant loss of DNA during normal Feulgen hydrolysis in the material used.

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