The Two-Wavelength Method of Microspectrophotometry | Zendy

Mortimer L. Mendelsohn | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The Two-Wavelength Method of Microspectrophotometry

Author(s) -

Mortimer L. Mendelsohn

Publication year - 1958

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.4.4.415

Subject(s) - absorbance , wavelength , chromophore , range (aeronautics) , table (database) , transmission (telecommunications) , relative standard deviation , optics , set (abstract data type) , product (mathematics) , field (mathematics) , biological system , materials science , biology , physics , mathematics , statistics , computer science , geometry , telecommunications , data mining , quantum mechanics , detection limit , pure mathematics , composite material , programming language

The calculations required for two-wavelength measurements are time consuming and laborious. In order to circumvent this limitation of the method, a set of tables which combined four operations into one has been designed and is reproduced within. The tables are based on Patau's formulae. The two transmission readings obtained according to the photometric method provide the coordinates which lead directly to a value for the relative absorbance. The product of this absorbance and the area of the photometric field gives the relative amount of chromophore in the field. The range of transmission values covered in the table corresponds to the effective range of the two-wavelength method.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research