Intracellular Distribution of 5' Nucleotidase in Rat Liver | Zendy

Gaston de Lamirande | Zendy; Claude Allard | Zendy; Antonio Cantero | Zendy

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Intracellular Distribution of 5' Nucleotidase in Rat Liver

Author(s) -

Gaston de Lamirande,

Claude Allard,

Antonio Cantero

Publication year - 1958

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.4.4.373

Subject(s) - 5' nucleotidase , biology , differential centrifugation , intracellular , microsome , centrifugation , biochemistry , nucleotidase , enzyme , nucleotide , microbiology and biotechnology , gene

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg(++) ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.

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