ISOLATION OF RIBOSOMES FROM CYSTS OF ENTAMOEBA INVADENS  | Zendy

Richard S. Morgan | Zendy; Henry S. Slayter | Zendy; David L. Weller | Zendy

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ISOLATION OF RIBOSOMES FROM CYSTS OF ENTAMOEBA INVADENS

Author(s) -

Richard S. Morgan,

Henry S. Slayter,

David L. Weller

Publication year - 1968

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.36.1.45

Subject(s) - ribosome , ribonuclease , biology , magnesium , biochemistry , microbiology and biotechnology , rna , chemistry , organic chemistry , gene

A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invaders is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the addition of manganous and calcium ions to the magnesium custom- arily employed. Reasons are given for equating these ribosomes with the particles of which the crystalline chromatoid bodies are made.

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