A CORRELATED HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDY OF THE INTRANUCLEAR CRYSTALLINE AGGREGATES OF ADENOVIRUS (RI-APC VIRUS) IN HELA CELLS | Zendy

David P. Bloch | Zendy; Councilman Morgan | Zendy; Gabriel C. Godman | Zendy; Calderón Howe | Zendy; Harry M. Rose | Zendy

Open Access

A CORRELATED HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDY OF THE INTRANUCLEAR CRYSTALLINE AGGREGATES OF ADENOVIRUS (RI-APC VIRUS) IN HELA CELLS

Author(s) -

David P. Bloch,

Councilman Morgan,

Gabriel C. Godman,

Calderón Howe,

Harry M. Rose

Publication year - 1957

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.3.1.1

Subject(s) - feulgen stain , biology , electron microscope , chromatin , hela , dna , osmium tetroxide , staining , virus , microbiology and biotechnology , cell nucleus , capsid , biophysics , negative stain , ultrastructure , virology , cell , nucleus , biochemistry , optics , anatomy , physics , genetics

HeLa cells in tissue cultures infected with types 3, 4, or 7 of adenovirus (RI-APC virus) were studied in order to correlate certain histochemical and electron microscopic findings. Adjacent thin (ca. 0.05 micro) and thick (2-4 micro) sections of osmium-fixed, methacrylate-embedded cells were cut; by mapping the sections the same cells could be identified with both the electron and the light microscope. Intranuclear crystalline aggregates seen with the electron microscope to be composed of ordered arrays of viral particles were found by means of the Feulgen reaction to contain DNA. DNA is therefore assumed to be a constituent of the viral particle. The virus appeared to develop from an osmiophilic Feulgen-negative matrix. Displacement of nuclear chromatin occurred during this process. A Feulgen-azure staining method was found to permit clear distinction between viral and nuclear (host) DNA in thick sections.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research