CYTOCHEMICAL STUDIES OF MITOCHONDRIA | Zendy

Michael L. Watson | Zendy; Philip Siekevitz | Zendy

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CYTOCHEMICAL STUDIES OF MITOCHONDRIA

Author(s) -

Michael L. Watson,

Philip Siekevitz

Publication year - 1956

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.2.6.639

Subject(s) - membrane , mitochondrion , biology , centrifugation , differential centrifugation , electron microscope , fraction (chemistry) , biochemistry , suspension (topology) , biophysics , chromatography , chemistry , physics , optics , mathematics , homotopy , pure mathematics

Mitochondria isolated from rat liver and suspended in 0.44 M sucrose were disrupted by treatment with 0.3 per cent Na deoxycholate. The treated suspension was fractionated by differential centrifugation into a number of fractions and the respective pellets were examined in sections in the electron microscope. One of these fractions was found to consist of apparently membrane-bound (vesicular) elements. The difference between interfaces and membranes was discussed and the material of this fraction was found to meet stated requirements identifying it as membranous. A detailed study of the disruption process undergone by mitochondria in the presence of Na deoxycholate showed that the elements of this fraction were derived from structural elements assumed to be mitochondrial membranes. The findings thus demonstrate that mitochondria do possess membranes as defined and that these membranes can be isolated in a relatively pure form.

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