AN IMMUNOCHEMICAL STUDY OF THE DISTRIBUTION OF MYOSIN IN GLYCEROL EXTRACTED MUSCLE | Zendy

Henry Finck | Zendy; Howard Holtzer | Zendy; John M. Marshall | Zendy

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AN IMMUNOCHEMICAL STUDY OF THE DISTRIBUTION OF MYOSIN IN GLYCEROL EXTRACTED MUSCLE

Author(s) -

Henry Finck,

Howard Holtzer,

John M. Marshall

Publication year - 1956

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.2.4.175

Subject(s) - biology , myosin , distribution (mathematics) , glycerol , microbiology and biotechnology , biochemistry , anatomy , mathematical analysis , mathematics

Recent investigations of the distribution of myosin and actin in the skeletal muscle fiber have relied largely on extraction techniques for the differential removal of specific proteins. This work has been reviewed by Hanson and Huxley (1). Because such extraction techniques may remove much soluble material in addition to myosin or actin, conclusions based on density changes within fibers after extractions have seemed somewhat questionable (2). We have, therefore, attempted a more positive localization of myosin in glycerol fibers by the use of the fluorescent antibody method (3, 4).

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