Open AccessA Method for the Histochemical Differentiation of Cholesterol and Its Esters
Author(s) -
Irwin Feigin
Publication year - 1956
Publication title -
the journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.414
H-Index - 380
eISSN - 1540-8140
pISSN - 0021-9525
DOI - 10.1083/jcb.2.2.213
Subject(s) - digitonin , cholesterol , biology , biochemistry , alcohol , ether , enzyme , organic chemistry , chemistry
Free cholesterol is demonstrated in formalin-fixed frozen sections when treated successively by digitonin, alcohol-ether, and the Schultz technique, in which circumstances cholesterol esters are not visualized. Cholesterol esters and free cholesterol are both demonstrated in comparable sections treated by the Schultz method alone, so that the difference between such sections indicates the sites at which cholesterol esters may be considered present.
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