THE USE OF SPECIFIC ANTIBODY IN ELECTRON MICROSCOPY | Zendy

Frank A. Pepe | Zendy

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THE USE OF SPECIFIC ANTIBODY IN ELECTRON MICROSCOPY

Author(s) -

Frank A. Pepe

Publication year - 1961

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.11.3.515

Subject(s) - fluorescein , conjugate , fluorescence microscope , electron microscope , antibody , staining , mercury (programming language) , biology , fluorescence , conjugated system , microscopy , antigen , biochemistry , biophysics , microbiology and biotechnology , chemistry , pathology , immunology , organic chemistry , optics , mathematical analysis , physics , genetics , mathematics , computer science , programming language , polymer , medicine

The preparation of antimyosin conjugated with mercury and fluorescein is described. The mercury was introduced to permit visualization of the antibody in the electron microscope. An organic mercurial, tetraacetoxymercuriarsanilic acid, was prepared and coupled to the antibody through the diazonium salt. The fluorescein was coupled through the isocyanate by a modification of the procedure described by Coons and Kaplan. The antibody conjugate retained its specificity of reaction with the tissue antigen. This was demonstrated by the staining pattern obtained in fluorescence microscopy.

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