A SIMPLE METHOD FOR OBTAINING INCREASED CONTRAST IN ARALDITE SECTIONS BY USING POSTFIXATION STAINING OF TISSUES WITH POTASSIUM PERMANGANATE | Zendy

D. F. Parsons | Zendy

Open Access

A SIMPLE METHOD FOR OBTAINING INCREASED CONTRAST IN ARALDITE SECTIONS BY USING POSTFIXATION STAINING OF TISSUES WITH POTASSIUM PERMANGANATE

Author(s) -

D. F. Parsons

Publication year - 1961

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.11.2.492

Subject(s) - potassium permanganate , araldite , biology , staining , contrast (vision) , differential staining , pathology , chemistry , genetics , medicine , adhesive , organic chemistry , layer (electronics) , artificial intelligence , computer science

Epoxy resin appears to be the best embedding material for preserving tissues for electron microscopy. Its low linear coefficient of contraction I ensures that no appreciable distortion can occur owing to polymerization damage or excessive shrinkage (1). This plastic also is exceptionally stable in the electron beam, so that surface damage to the section is slight. Although Araldite blocks are often more difficult to cut than those of methacrylate, the introduction of the diamond knife (2) with its superior cutting abilities over

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